FIG 3.

Effect of LT gene deletion in S. aureus MR10 and USA300. (A) Biofilm formation of wild-type (WT) and LT mutant. Biofilms formed on the bottom of the 96-well plate were stained with crystal violet; subsequently, absorbance at 595 nm was measured. The values are the means of six independent cultures. The error bars indicate the standard error of the mean. Differences between WT and mutants were compared using Student’s t test. ***P < 0.001. (B) Western blot showing levels of PBP2a in WT and LT mutants. Concentrations of oxacillin used for induction are indicated above the panels. As loading controls, CBB-stained gels of the same area are shown below the panels.