TABLE 2.
MICs of antibiotics for WT and LT mutants of S. aureus MR23 cultured in Mueller-Hinton brotha
| Antibiotic | MIC (μg/ml) of: |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WT | ΔisaA | ΔsceD | ΔisaAΔsceD | WT_EV | WT_pCNisaA | WT_pCNsceD | ΔisaA_EV | ΔisaA_pCNisaA | ΔisaA_pCNsceD | ΔsceD_EV | ΔsceD_pCNsceD | |
| β-lactam | ||||||||||||
| Oxacillin | 64 | 1 | 32 | 1 | 64 | 64 | 32 | 2 | 16 | 2 | 64 | 64 |
| Ampicillin | 4 | 2 | 0.5 | 0.5 | 8 | 8 | 8 | 4 | 8 | 4 | 0.5 | 8 |
| Cefazolin | 8 | 1 | 8 | 1 | 16 | >16 | >16 | 1 | 16 | 2 | 1 | >16 |
| Cefmetazole | 8 | 4 | 8 | 2 | 8 | >32 | >32 | 4 | 16 | 4 | 8 | 32 |
| Flomoxef | 8 | 2 | 4 | 1 | 8 | >16 | >16 | 1 | 8 | 2 | 4 | >16 |
| Cefoxitin | >16 | 8 | 8 | 8 | >16 | >16 | >16 | 8 | >16 | 16 | 8 | >16 |
| Imipenem | 8 | <0.25 | 8 | <0.25 | 8 | >8 | >8 | <0.25 | 8 | <0.25 | 8 | 8 |
| Glycopeptide | ||||||||||||
| Vancomycin | 1 | 2 | 1 | 2 | 2 | 4 | 4 | 2 | 4 | 2 | 1 | 2 |
| Teicoplanin | 4 | 4 | 4 | 2 | 1 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| Oxazolidinone | ||||||||||||
| Linezolid | 8 | 8 | 8 | 2 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| Lincomycin | ||||||||||||
| Clindamycin | 0.5 | 0.5 | 0.12 | 0.25 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.12 | 0.5 |
a
WT, wild type; ΔisaA, isaA-deleted strain; ΔsceD, sceD-deleted strain; ΔisaAΔsceD, isaA and sceD-deleted strain; EV, pCN51Cm (empty vector); pCNisaA, pCN51Cm containing isaA; pCNsceD, pCN51Cm containing sceD.