TABLE 1.
MICs and interpretations for antimicrobials tested against 2016AM-0877, E. coli TOP10, and transformant TOP10-pM216_mF
| Antimicrobial | MIC (interpretation) (μg/ml) for straina: |
||
|---|---|---|---|
| 2016AM-0877 | TOP10 | TOP10-pM216_mF | |
| Amoxicillin-clavulanic acid | 32 (R) | NA | NA |
| Ampicillin | >32 (R) | NA | NA |
| Azithromycin | 1 (WT) | NA | NA |
| Cefoxitin | 8 (S) | NA | NA |
| Ceftriaxone | ≤0.25 (S) | NA | NA |
| Chloramphenicol | >32 (R) | NA | NA |
| Ciprofloxacin | >4 (R) | ≤0.0039 (S) | 0.031 (S) |
| Levofloxacin | NA | 0.0078 (S) | 0.016 (S) |
| Gentamicin | 1 (S) | NA | NA |
| Meropenem | ≤0.06 (S) | NA | NA |
| Nalidixic acid | >32 (R) | NA | NA |
| Streptomycin | >64 (R) | NA | NA |
| Sulfisoxazole | ≤16 (S) | NA | NA |
| Tetracycline | >32 (R) | NA | NA |
| Trimethoprim-sulfamethoxazole | 0.25 (S) | NA | NA |
a
The interpretation criteria used to categorize MIC results as susceptible (S), resistant (R), and wild type (WT) are based on current breakpoints provided by the Clinical and Laboratory Standards Institute (CLSI) or MIC distribution data collected by the National Antimicrobial Resistance Monitoring System. Experiments were performed in triplicate. NA, not assessed.