FIG 4.

Detection of different concentrations of BlaC (0.1 to 500 nM) with fluorogenic probes CDG-1, CDG-OMe, and CDG-3 at 1 μM in MES buffer, pH 6.0 (A); sputum alone (B); and sputum with transport stabilization solution (TSS) (C) at room temperature (25°C) for 3 h. Data points shown represent the average fluorescence from samples done in triplicate, and error bars represent the standard deviations for these data. P values are shown above data points and are derived from comparison of data for CDG-3 with that of CDG-OMe by ANOVA.