TABLE 1.
Specificity of substrates for β-lactamases
| β-lactamase | CDG-OMe activity (%)a | CDG-3 activity (%)b |
|---|---|---|
| BlaCc | 100 ± 9.29 | 100 ± 2.00 |
| TEM-1d | 5.74 ± 0.55 | 3.44 ± 0.08 |
| AmpCd | 26.7 ± 2.66 | 6.82 ± 0.28 |
| KPC-2d | 53.3 ± 3.28 | 17.9 ± 1.07 |
| CTX-M-15e | 5.91 ± 0.53 | 2.79 ± 0.39 |
| P99d | 7.22 ± 0.75 | 2.15 ± 0.13 |
a
Activity was calculated as the percentage of fluorescence (excitation, 490 nm; emission, 515 nm) after coincubation of 0.2 nM of each β-lactamase with 1 μM CDG-OMe for 40 min at room temperature (25°C).
b
Activity was calculated in exactly the same manner as for CDG-OMe, except that 1 μM CDG-3 was used instead of CDG-OMe.
c
P < 0.0001 for BlaC compared to all other β-lactamases for both CDG-OMe and CDG-3.
d
P < 0.01 for CDG-3 compared to CDG-OMe for this β-lactamase.
e
P < 0.05 for CDG-3 compared to CDG-OMe for this β-lactamase.