TABLE 1.
Reproducibility of the IgG capture assay
| Sample | Mean S/CO (SD)a | CV%b |
|---|---|---|
| Intra-assay variability | ||
| Plasma | ||
| HTLV-1 sample A | 21.84 (0.69) | 3.1% |
| HTLV-2 sample B | 3.05 (0.09) | 2.9% |
| Oral fluid | ||
| Pool with low S/CO | 5.55 (0.15) | 2.65% |
| Pool with high S/CO | 20.91 (0.84) | 4% |
| Interassay variability | ||
| Plasma | ||
| HTLV-1 sample A | 21.63 (1.39) | 6.4% |
| HTLV-1 sample B | 7.25 (0.64) | 8.9% |
| HTLV-1 sample C | 21.27 (0.93) | 4.4% |
| HTLV-1 sample D | 19.73 (1.17) | 5.9% |
| HTLV-1 sample E | 8.73 (0.94) | 10.8% |
| HTLV-1 sample F | 6.39 (0.33) | 5.2% |
| HTLV-1 sample G | 5.35 (0.35) | 6.5% |
| HTLV-2 sample A | 2.96 (0.03) | 1.1% |
| Oral fluid | ||
| Pool with low S/CO | 6.05 (0.53) | 8.7% |
| Pool with high S/CO | 22.36 (1.5) | 6.7% |
a
S/CO, signal-to-cutoff ratio; SD, standard deviation.
b
CV%, coefficient of variation.