Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Drosophila melanogaster)	w; elav-GAL4, tsh-GAL80/ +,+; cha3.3-GAL80, UAS-EGFP/ +	DOI: 10.1016/j.cub.2012.07.048	NA	BL/+
Strain, strain background (Drosophila melanogaster)	w; elav-GAL4, tsh-GAL80/ +,+; cha3.3-GAL80, UAS-EGFP / +	DOI:10.1016/j.cub.2012.07.048	NA	BL > +
Strain, strain background (Drosophila melanogaster)	w; elav-GAL4, tsh-GAL80/ +,+; UAS-EGFP / +	DOI: 10.1016/j.cub.2012.07.048	NA	BLsens > +
Strain, strain background (Drosophila melanogaster)	20XUAS-IVS-Syn21-Shibire-ts1-GFP-p10	Gift from G. Rubin	NA
Strain, strain background (Drosophila melanogaster)	MB247-GAL4	Gift form J. Ng	NA
Strain, strain background (Drosophila melanogaster)	UAS-rpr, UAS-hid	Gift from from M. Landgraf	NA
Strain, strain background (Drosophila melanogaster)	UAS-myrRFP	Gift from from M. Landgraf	NA
Strain, strain background (Drosophila melanogaster)	P{GMR57C10-GAL4}attP2	Bloomington Drosophila Stock Center	RRID:BDSC_39171	In attp40
Strain, strain background (Drosophila melanogaster)	P{tubP-GAL80[ts]}2	Bloomington Drosophila Stock Center	RRID:BDSC_7017
Chemical	Poly-L-Lysine hydrobromide	Sigma	P2524
Antibody	Mouse monoclonal anti-SCR	Development Studies Hybridoma Bank (DSHB), IA, USA	RRID:AB_528462	1: 20 dilution
Antibody	Chicken polyclonal anti-GFP	abcam	ab13970	1/2000 dilution
Antibody	Rabbit polyclonal anti-Cleaved Drosophila Dcp-1(Asp216)	Cell Signaling Technology	#9578	1/100
Antibody	Donkey polyclonal Alexa568 anti-mouse	Invitrogen	A10042	1/500
Antibody	Donkey polyclonal CF633 anti-rabbit	Biotum	BT20125	1/500 dilution
Antibody	Goat polyclonal Alexa488 anti-chicken	Biotum	BT20020	1/500
Software	FIMTrack	https://www.uni-muenster.de/PRIA/en/FIM/download.shtml	NA	FIMTrack_v2_X64_MacOS
Software	MBA MLE Analysis	http://dx.doi.org/10.1111/2041-210X.12096	NA
Software	clampfit	https://moleculardevices.app.box.com/s/l8h8odzbdikalbje1iwj85x88004f588	NA