Figure 6.

Molecular definition of AA and its subdomains. (a) Drawing depicts structures in sagittal images (a’-a” and c-h). (a’-a’’) In situ hybridization images define AA and its subdomains AAc, AArl and AAv based on SCUBE1 (a’) and AQP1 (a’’); AIrv in (a) indicates region of moderate HTR1B expression caudal to AAc (seen in h). (b) Drawing depicts structures in transverse images (b’-b’’). (b’-b’’) In situ hybridization images define AA and its subdomains AAc, AArm, AArl and AAv based on SCUBE1 (b’) and AQP1 (b’’). In both (a) and (b), thick dashed lines represent SCUBE1 expression, thin dashed lines represent AQP1 expression, grey patches indicate fiber bundles, diagrams on upper right indicate positions of the drawn areas relative to reference sections, and coordinates (in mm) indicate the position along the respective axes. (c-h) In situ hybridization sagittal images for positive (c-e/g-h: SCUBE1, PLPP4, CRHR2, CAMK2N1 and HTR1B) and negative (f: ETV1) markers of AA and its subdomains; (c) is a repeat of (a’) for contrast with other genes. For abbreviations, see list. Scale: 250 μm.