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Maximum turnover numbers (kcat) for thiosulfate oxidation (red) and tetrathionate reduction (blue) by Av and Cj WT TsdA enzymes, and the Cj N254K variant, as determined by in vitro spectrophotometric enzyme activity assays using ferricyanide as an electron acceptor for thiosulfate oxidation and reduced methyl viologen as an electron donor for tetrathionate reductions. Adapted from Refs. 4 and 5.
