Figure 2.

Effects of PLK1 inhibition in As₂O₃-treated cells. A, HaCaT cells were treated for 24 h with the indicated amount of arsenite, and then cells were collected, and the cell cycle was analyzed by FACS. B–D, immortalized HaCaT cells were treated with the indicated amount of As₂O₃ (AS) for 24 h, and then cells were treated with/without the indicated inhibitors (PLK1 inhibitor BI2536; ZM447439 Aurora A/B; RO3280 PLK1) for 24 h and analyzed by immunoblotting with the indicated antibodies. E, the indicated cell lines were treated with As₂O₃ for 24 h, and then cells were treated with/without 10 μm ZM447439 (ZM) for 24 h and analyzed by immunoblotting with the indicated antibodies. F, immortalized (HaCaT-S) and As₂O₃-transformed HaCaT cells (HaCaT-R) were treated with increasing amounts of As₂O₃ for 24 h and analyzed by immunoblotting with the indicated antibodies. Shown are representative results from at least three independent experiments. UN, untreated.