Figure 7.

NOTCH1-dependent increased expression of IL-6 and IL-8 during DNA damage–induced growth arrest. SCCO22 cells were treated with doxorubicin (DOX), following which either DMSO or GSI was added, and cells were maintained in culture for a further 24 h. In A, cells were analyzed by FACS analysis. B, cells were treated as described in A, and whole-cell lysate was used for Western blotting with the indicated antibodies. Additional samples present on the gel were cropped as indicated by dashed lines. In C, cells were treated as described for A, and total RNA was used for quantitative RT-PCR with the indicated probe.