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. 2019 Oct 8;294(47):17735–17757. doi: 10.1074/jbc.RA119.007635

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© 2019 Kirmiz et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 1. — Membrane-associated phosphatidylinositol transfer proteins biochemically associate with Kv2.1 in mouse brain. A, total spectra counts of proteins recovered from a single trial of Kv2.1 or Kv1.2 IPs from WT mouse brain and summed spectra counts from three separate trials of Kv2.1 IPs from WT or Kv2.1 KO mouse brain. Note the enriched recovery of Kv2.2, VAPA and VAPB, and Nir2/3 in the Kv2.1 IPs relative to the Kv1.2 IPs. Note the nonspecific recovery of ROA2 and GRP75 in all samples. B, multiplexed immunoblot analysis of input and output fractions from a single trial of Kv2.1 or Kv1.2 IPs performed using WT mouse brain. Note the presence of both Kv2.1 (cyan band, ≈110 kDa) and Kv1.2 (magenta band, ≈70 kDa) in the input fraction and the exclusive presence of Kv2.1 in the Kv2.1 IP output fraction, and Kv1.2 in the Kv1.2 IP output fraction.