Table 2.

Quantification of the impact of Kv2.1 signal segmentation and randomization using various block sizes on overlap with Nir2

NA means not applicable.

Sample	Percent of total Nir2 signal overlapping with Kv2.1	S.D.	p value (two-tailed, paired t-test vs. percent overlap in original image)
Original image	23.9	5.97	NA
Kv2.1 randomized (1 × 1 pixel block size)	1.99	0.437	0.000119
Kv2.1 randomized (5 × 5 pixel block size)	1.95	0.426	0.0000303
Kv2.1 randomized (10 × 10 pixel block size)	1.93	0.458	0.0000174
Kv2.1 randomized (20 × 20 pixel block size)	1.84	0.398	0.0000176
Kv2.1 randomized (30 × 30 pixel block size)	1.74	0.399	0.0000267
Kv2.1 randomized (40 × 40 pixel block size)	1.68	0.380	0.0000222
Kv2.1 randomized (50 × 50 pixel block size)	1.65	0.405	3.76E-06