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APP cell surface localization is not affected by APP–Ser-675 phosphorylation. A, representative Western blot analysis of total APP in cell lysate and pulled down biotinylated APP from APPwt (lanes 2 and 6), APP-S675A (lanes 3 and 7), and APP-S675E (lanes 4 and 8) overexpressing SK-N-AS cells. B, relative abundance of biotinylated full-length APP from APPwt, APP-S675A, and APP-S675E overexpressing SK-N-AS cells. n.s, not significant; n = 4.
