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. 2019 Oct 11;294(47):17768–17776. doi: 10.1074/jbc.RA119.008310

Figure 5.

Figure 5.

Meprin β knockdown decreases formation of the slower migrating CTF in APP-S675E cells, without affecting the total level of APP-CTFs. A, representative blot showing the expression of full-length APP, APP-CTFs, and meprin β in DAPT (γ-secretase inhibitor)-treated, non–APP-transfected (lanes 1 and 2), APPwt (lanes 3 and 4), or APP-S675E (lanes 5 and 6) overexpressing SK-N-AS cells co-transfected with meprin β (lanes 2, 4, 6) or control (lanes 1, 3, 5) siRNA. B, quantification of meprin β expression in SK-N-AS cells transfected with control or meprin β siRNA. C, quantification of full-length APP, normalized against tubulin, in cells treated as in A. D, quantification of the total APP-CTF levels, normalized against the corresponding APP full-length level, in cell lysate from APPwt and APP-S675E overexpressing SK-N-AS cells treated with meprin β or control siRNA. E, quantification of the upper slower C99 CTF, normalized against the corresponding APP full-length level, in cell lysate from APPwt and APP-S675E overexpressing SK-N-AS cells treated with meprin β or control siRNA. F, ratio of APP-CTF upper/APP-CTF lower band in cell lysate of APPwt and APP-S675E overexpressing SK-N-AS cells, treated with meprin β or control siRNA. For quantifications, *, p < 0.05; **, p < 0.01; ***, p < 0.001; n = 4.