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. 2019 Oct 17;294(47):17863–17874. doi: 10.1074/jbc.RA119.010284

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© 2019 Zhang et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — Linc/circRNAs sponge miRNAs targeting on Oct4. A, expression patterns of linc/circRNAs, which have complementary binding sites with the four Oct4-miRNAs. B, fold-change of Oct4 mRNA with knockdown of the linc/circRNAs as indicated. Down-regulation of the linc/circRNAs highlighted in red significantly suppressed Oct4 expression by at least 20% (p < 0.001). C, RIP assay for detecting the interaction of the Oct4-miRNAs with the specific linc/circRNAs. Biotin-labeled miRNAs were transfected into MEFs at a final concentration of 20 nm, and the RIP assay was performed using day 3 rMEFs (n = 3). The enrichment of lincRNAs or circRNAs in bound fractions was evaluated by qRT-PCR analysis normalized to Oct4 mRNA. Gapdh was analyzed as the negative control. D, subcellular localization analysis of the linc/circRNAs. Nuc, nucleoplasm; Cyto, cytoplasm. Gapdh and Xist act as cytoplasmic and nuclear control, respectively.