Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Characterization of BcelPL6 mutant enzymes. A, kinetic data of WT and mutants normalized to WT values for A: top, alginate; bottom, polyM. kcat (black), Km (red), and kcat/Km (blue). B, melting temperature of WT and mutants determined by DSC. The black line represents WT melting temperature. C, imidazole rescue of activity of BcelPL6 H271N toward 4 mg ml−1 alginate.
