FIG. 12.

(a) The general phase diagram predicted by the Flory-Huggins model indicating the proteins’ affinity to the L_d phase, L_o phase, or phase boundary (bo). At the red (blue) surfaces, the protein concentration of the L_d (L_o) phase is equal to the protein line density at the phase boundary. When χ is small and/or T is high, the miscible phase will form (under gray surface, calculated for N $\to$ $\infty$) (b) The χ and η values obtained with protein monomer simulations are displayed on the phase diagram at T = 295 K. The black solid and black dashed lines show the χ value obtained from the current CWT result from lipid-only simulation and one that can be obtained in spirit of our previous work,²⁴ respectively. As discussed in the main text, the deviation of χ obtained with protein monomer simulation from that obtained with a lipid-only simulation (black lines) can be thought of the protein’s affinity to the boundary. The gray line corresponds to the gray surface in panel (a).