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. 2019 Nov 22;39(11):BSR20192236. doi: 10.1042/BSR20192236

Figure 5. CTS ameliorates thrombin-induced endothelial inflammation via TLR-4-p38 signaling.

Figure 5

(A) HPAECs were pretreated with CTS (50 nmol/l) for 30 min and then stimulated with thrombin (1 U/ml) for another 12 h. The levels of VWF (A), P-selectin (B), and E-selectin (C) in endothelial cell supernatant were determined by ELISA. (D,E) The protein expression of TLR-4 (D) and the phosphorylation of p38 (E) were determined by Western blotting. **P<0.01 vs. control untreated; ##P<0.01 vs. thrombin untreated, n=6. (F–H) The CTS-pretreated cells were treated with thrombin and Lacz adenovirus or TLR-4 adenovirus (AdTLR-4, 50 MOI) for 24 h. The levels of VWF (F), P-selectin (G), and E-selectin (H) in endothelial cell supernatant. **P<0.01 vs. control; ##P<0.01 vs. thrombin; &&P<0.01 vs. thrombin+CTS, n=6.