Table 2. Details of primers of candidate control genes and validation genes and parameters derived from qPCR analysis.
| Gene symbol | Prime Sequence Forward/reverse | Amplicon length (bp)* | E (%)** | R2** | |
|---|---|---|---|---|---|
| cDNA | gDNA | ||||
| ACT | AGGCAGGATTTGCTGGTGATGATGCT/ATACGCATCCTTCTGTCCCATTCCGA | 107 | 107 | 102.27 | 0.982 |
| CAC | CCTCCGTTGTGATGTAACTGG/ATTGGTGGAAAGTAACATCATCG | 173 | 592 | 95.88 | 0.992 |
| EXPRESSED | GCTAAGAACGCTGGACCTAATG/TGGGTGTGCCTTTCTGAATG | 183 | 291 | 91.7 | 0.996 |
| GAPDH | GGCTGCAATCAAGGAGGAA/AAATCAATCACACGGGAACTG | 204 | N/A | 94.22 | 0.998 |
| PP2a | TGGCAAAGGTGTTGCAATCC/CTTTCCCCTTTTGCTTCTTCGTG | 266 | 450 | 99.57 | 0.996 |
| RPL8 | CCGAAGGAGCTGTTGTTTGTA/ACCTGACCAATCATAGCACGA | 184 | 1091 | 101.89 | 0.994 |
| SAND | TTGCTTGGAGGAACAGACG/GCAAACAGAACCCCTGAATC | 164 | 3559 | 107.09 | 0.989 |
| TIP41 | ATGGAGTTTTTGAGTCTTCTGC/GCTGCGTTTCTGGCTTAGG | 235 | N/A | 99 | 0.995 |
| FUL1 | GTTTTGCCACAACAACTGGACTC/CTTGCTGCTGTGAAGAACTACC | 106 | 1124 | 98.94 | 0.997 |
| AP2c | CCGTTTCGAATTCAAGTTCA/ACCCAGACCCACCATAGAGA | 122 | 122 | 107.25 | 0.981 |
| SlYABBY1a | ACCCAAATGAATCACTCATGCCA/CTTTGATACGTTGGATCTCGTCCT | 144 | N/A | 104.04 | 0.985 |
| SlYABBY2a | TCTGCAGCACAATTCTTGCG/AATTTGCGCAGTGTCCACAC | 84 | 2346 | 102.8 | 0.988 |
*Estimated form Micro-Tom cDNA/gDNA sequences in TOMATOMICS (http://bioinf.mind.meiji.ac.jp/tomatomics/index.php). **Mean of 3 biological replicates, N/A=Non-amplified.