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. 2018 Sep 25;35(3):259–266. doi: 10.5511/plantbiotechnology.18.0613a

Figure 3. Proanthocyanidin accumulation in Ipomoea seeds. (A) Temporal accumulation of proanthocyanidins in wild-type I. nil (TKS). Numerals on photos indicate days after pollination (DAP); seeds were soaked in a vanillin or DMACA solution for staining. (B) Proanthocyanidin accumulation in immature (28 DAP) seeds of anthocyanin-deficient I. nil mutants. The lines used were TKS (wild-type), AK19 (CHS-D mutant), AK40 (CHI mutant), AK10 (DFR-B mutant), AK23 (ANS mutant), NS/W1ca1 (InWDR1 mutant). Flower and seed phenotypes of these lines are shown in Figure 2. (C) Proanthocyanidin accumulation in seed coat of the CHS-D mutant I. purpurea line (PR640) at 28 DAP. Lines FP39 (wild-type) and YJH/WR-4 (bHLH2 mutant) were used as positive and negative controls, respectively. (D) Proanthocyanidin mutant screening revealed that brown mutant lines Q0306 and Q1041 were proanthocyanidin mutants. (E) Immature seeds at various developmental stages of wild-type I. quamoclit (Q0055) remained unstained after being soaked in a DMACA solution.

Figure 3. Proanthocyanidin accumulation in Ipomoea seeds. (A) Temporal accumulation of proanthocyanidins in wild-type I. nil (TKS). Numerals on photos indicate days after pollination (DAP); seeds were soaked in a vanillin or DMACA solution for staining. (B) Proanthocyanidin accumulation in immature (28 DAP) seeds of anthocyanin-deficient I. nil mutants. The lines used were TKS (wild-type), AK19 (CHS-D mutant), AK40 (CHI mutant), AK10 (DFR-B mutant), AK23 (ANS mutant), NS/W1ca1 (InWDR1 mutant). Flower and seed phenotypes of these lines are shown in Figure 2. (C) Proanthocyanidin accumulation in seed coat of the CHS-D mutant I. purpurea line (PR640) at 28 DAP. Lines FP39 (wild-type) and YJH/WR-4 (bHLH2 mutant) were used as positive and negative controls, respectively. (D) Proanthocyanidin mutant screening revealed that brown mutant lines Q0306 and Q1041 were proanthocyanidin mutants. (E) Immature seeds at various developmental stages of wild-type I. quamoclit (Q0055) remained unstained after being soaked in a DMACA solution.