Table 2. Flavonoid biosynthesis genes found in the Ipomoea nil genome.

Gene	Accession No.	Gene ID		RNA expression		RNA-seq		Mutation	Necessity**
		Original*	NCBI	Petal	Seed coat	Flower	Seed coat		Petal	Seed coat
CHS-D	AB001818	INIL12g08537	109176547	+	−	+	−	transposon	essential	unnecessary
CHS-E	AB001819	INIL14g35461	109161594	+	+	+	+		essential***	essential
CHI		INIL11g18443	109186979	+	+	+	+	transposon	essential	not essential
F3H-A	D83041	INIL02g39928	109167724	+	+	+	+		(redundant)	(redundant)
F3H-B		INIL02g39929	109167725	+	+	+	+	pseudo gene	unnecessary	unnecessary
F3H-C		INIL05g09512	109177773	+	+	+	+		(redundant)	(redundant)
F3′H	AB113261	INIL04g34064	109160581	+	+	+	+	substitution	not essential	not essential
DFR-A		INIL05g09558	109177611	+	+	+	−		unnecessary	redundant
DFR-B	AB006792	INIL05g09559	109177619	+	+	+	−	transposon	essential	not essential
DFR-C		INIL05g09560	109177620	+	−	+	−		unnecessary	unnecessary
ANS	AB073920	INIL13g40254	109168138	+	−	+	−	transposon	essential	unnecessary
3GT	LC019108	INIL10g12321	109182028	+	+	+	+	transposon	essential	(unnecessary)

*The original gene ID is from a previous publication (Hoshino et al. 2016). **Predictions without mutant analyses are presented in parentheses. ***CHS-E expression is responsible for flower tube pigmentation in some I. nil lines (Hoshino et al. 2009).