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. 2018 Sep 25;35(3):273–279. doi: 10.5511/plantbiotechnology.18.0508a

Figure 3. Expression of genes encoding cell-wall loosening proteins in Arabidopsis. Expression level of AtEXPA1 (At1g69530), AtEXPA2 (At5g05290), AtEXPA6 (At2g28950), AtEXPB1 (At2g20750), AtXTH4 (At2g06850) and AtXTH23 (At4g25810) was investigated in 2-week-old seedlings of wild-type Columbia, p35S:OsAP2 and p35S:OsWRKY24 plants. qPCR was performed on a CFX96TM real-time system (Bio-Rad) using Maxima SYBR Green qPCR Master Mix (Thermo). The primers used for quantification are listed in Supplementary Table S1. Actin (Act) was used as a standard for quantification of gene expression. Data are represented as mean±SD of three biological replicates.

Figure 3. Expression of genes encoding cell-wall loosening proteins in Arabidopsis. Expression level of AtEXPA1 (At1g69530), AtEXPA2 (At5g05290), AtEXPA6 (At2g28950), AtEXPB1 (At2g20750), AtXTH4 (At2g06850) and AtXTH23 (At4g25810) was investigated in 2-week-old seedlings of wild-type Columbia, p35S:OsAP2 and p35S:OsWRKY24 plants. qPCR was performed on a CFX96TM real-time system (Bio-Rad) using Maxima SYBR Green qPCR Master Mix (Thermo). The primers used for quantification are listed in Supplementary Table S1. Actin (Act) was used as a standard for quantification of gene expression. Data are represented as mean±SD of three biological replicates.