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. 2018 Sep 25;35(3):273–279. doi: 10.5511/plantbiotechnology.18.0508a

Figure 4. Subcellular localization of OsAP2 and OsWRKY24 in Arabidopsis mesophyll cells. (A) YFP:OsAP2 and nuclear localization signal (NLS):mCherry were co-transformed into Arabidopsis protoplasts. YFP signals from YFP:OsAP2 are exclusively localized in the nucleus. (B) YFP:OsWRKY24 and NLS:mCherry were co-transformed into Arabidopsis protoplasts. YFP signals from YFP:OsWRKY24 form nuclear speckles. Red florescence is from a nuclear marker, NLS:mCherry (Lee et al. 2012). Isolation and transfection of Arabidopsis protoplasts followed, as described by Wu et al. (2009). Bar=10 µm.

Figure 4. Subcellular localization of OsAP2 and OsWRKY24 in Arabidopsis mesophyll cells. (A) YFP:OsAP2 and nuclear localization signal (NLS):mCherry were co-transformed into Arabidopsis protoplasts. YFP signals from YFP:OsAP2 are exclusively localized in the nucleus. (B) YFP:OsWRKY24 and NLS:mCherry were co-transformed into Arabidopsis protoplasts. YFP signals from YFP:OsWRKY24 form nuclear speckles. Red florescence is from a nuclear marker, NLS:mCherry (Lee et al. 2012). Isolation and transfection of Arabidopsis protoplasts followed, as described by Wu et al. (2009). Bar=10 µm.