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. 2018 Sep 25;35(3):273–279. doi: 10.5511/plantbiotechnology.18.0508a

Figure 5. Transgenic Arabidopsis plants overexpressing a chimeric repressor of OsWRKY24 (OsWRKY24-SRDX). The OsWRKY24-SRDX DNA fragment for an entry clone was amplified by PCR using the following primers, 5′GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CAT GAC AAC CTC GTC GTC3′ and 5′GGG GAC CAC TTT GTA CAA GAA AGC TGG GTG CTA TGC GAA TCC TAG TTC CAG TTC GAG ATC CAG GTC TAG GTA GAG CGA GTT CTG3′. Transgenic plants containing p35S:OsWRKY24-SRDX construct with high expression level of the transgene exhibit an indistinguishable phenotype to WT.

Figure 5. Transgenic Arabidopsis plants overexpressing a chimeric repressor of OsWRKY24 (OsWRKY24-SRDX). The OsWRKY24-SRDX DNA fragment for an entry clone was amplified by PCR using the following primers, 5′GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CAT GAC AAC CTC GTC GTC3′ and 5′GGG GAC CAC TTT GTA CAA GAA AGC TGG GTG CTA TGC GAA TCC TAG TTC CAG TTC GAG ATC CAG GTC TAG GTA GAG CGA GTT CTG3′. Transgenic plants containing p35S:OsWRKY24-SRDX construct with high expression level of the transgene exhibit an indistinguishable phenotype to WT.