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. 2018 Jun 25;35(2):141–154. doi: 10.5511/plantbiotechnology.18.0427a

Figure 6. Roles of WOX1/PRS and auxin in leaf development. (A) Phenotype of the aerial part of wild type, prs, wox1 and prs wox1 in the presence of 10 µM NPA. Leaf initiation defects in 10 µM NPA-treated prs and prs wox1 plants. (B) Short pin-like phenotype of the 10 µM NPA-treated prs shoot apex. (C) Phenotype of wild type and the FILp::WOX1 plants treated with 10 µM NPA. (D) Leaf phenotype of 1 µM 2,4-D-treated FILp::WOX1 plants. The arrows indicate the fusion of petioles between two leaves observed in wild type. The arrow heads indicate cup-shaped leaves observed in the FILp::WOX1 plants. Plants shown in (A–D) were grown on a solid medium. (E) Summary of the leaf phenotypes of mock-, NPA- or 2,4-D-treated plants. Schematic diagram of the vicinity of the shoot apical meristem observed from above. “M” represents the center of the meristem, “PZ” represents the peripheral zone, and “N.E”. represents “not examined”. Arrows indicate the degree and direction of growth. Scale bars, 4 mm (A), 200 µm (B), 1 cm (C), 1 mm (D).

Figure 6. Roles of WOX1/PRS and auxin in leaf development. (A) Phenotype of the aerial part of wild type, prs, wox1 and prs wox1 in the presence of 10 µM NPA. Leaf initiation defects in 10 µM NPA-treated prs and prs wox1 plants. (B) Short pin-like phenotype of the 10 µM NPA-treated prs shoot apex. (C) Phenotype of wild type and the FILp::WOX1 plants treated with 10 µM NPA. (D) Leaf phenotype of 1 µM 2,4-D-treated FILp::WOX1 plants. The arrows indicate the fusion of petioles between two leaves observed in wild type. The arrow heads indicate cup-shaped leaves observed in the FILp::WOX1 plants. Plants shown in (A–D) were grown on a solid medium. (E) Summary of the leaf phenotypes of mock-, NPA- or 2,4-D-treated plants. Schematic diagram of the vicinity of the shoot apical meristem observed from above. “M” represents the center of the meristem, “PZ” represents the peripheral zone, and “N.E”. represents “not examined”. Arrows indicate the degree and direction of growth. Scale bars, 4 mm (A), 200 µm (B), 1 cm (C), 1 mm (D).