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. 2018 Jun 25;35(2):101–111. doi: 10.5511/plantbiotechnology.18.0308a

Figure 2. K+ transport activity of rice cluster I HAK transporters. (A) Complementation of a yeast strain defective in high-affinity K+ uptake with HAK transporters belonging to cluster I. The trk1 trk2 yeast cells carrying empty vector pYES2 or the vector containing rice HAK cDNA were grown on arginine phosphate medium supplemented with 10, 0.3, or 0.1 mM KCl. Images of the cultures were obtained after incubation for 3 (10 mM KCl) or 7 (0.3, 0.1 mM KCl) days at 30°C. (B) Complementation of an E. coli strain defective in K+ uptake with the four HAK transporters belonging to cluster I. E. coli strain (LB 2003), deficient in the K+ transport system, was transformed with an empty vector or with the vector containing OsHAK cDNAs under the control of an IPTG-responsive promoter. Ten-fold dilutions of cells were inoculated, as shown on the upper side. Images of the cultures were obtained after incubation for 2 (30 mM KCl) or 3 (5 mM KCl) days at 30°C.

Figure 2. K+ transport activity of rice cluster I HAK transporters. (A) Complementation of a yeast strain defective in high-affinity K+ uptake with HAK transporters belonging to cluster I. The trk1 trk2 yeast cells carrying empty vector pYES2 or the vector containing rice HAK cDNA were grown on arginine phosphate medium supplemented with 10, 0.3, or 0.1 mM KCl. Images of the cultures were obtained after incubation for 3 (10 mM KCl) or 7 (0.3, 0.1 mM KCl) days at 30°C. (B) Complementation of an E. coli strain defective in K+ uptake with the four HAK transporters belonging to cluster I. E. coli strain (LB 2003), deficient in the K+ transport system, was transformed with an empty vector or with the vector containing OsHAK cDNAs under the control of an IPTG-responsive promoter. Ten-fold dilutions of cells were inoculated, as shown on the upper side. Images of the cultures were obtained after incubation for 2 (30 mM KCl) or 3 (5 mM KCl) days at 30°C.