TABLE 2.
Primers sequences for RT-qPCR.
| Genes | Primer sequence (5′-3′) | Product (bp) | GenBank accession |
| TLR1 | F:TTAGGAGACTCTTACGGGGAA | 135 | NM_001031775.1 |
| R:ATTTACTGCGGTGCTGACTGA | |||
| TLR2 | F:GTTTTACGGAAATTGTGAAACTG | 128 | NM_213761.1 |
| R:TCCACATTACCGAGGGATTT | |||
| TLR3 | F:GCATTGCCTGGTTTGTTAGTTG | 122 | NM_001097444.1 |
| R:TGTATCAAAAAGAATCACTGGGAG | |||
| TLR4 | F:ATATGGCAGAGGTGAAAGCAC | 125 | NM_001113039.2 |
| R:GAAGGCAGAGATGAAAAGGGG | |||
| TLR5 | F:AGTTCCGGGGATTTTGTTTCA | 110 | NM_001348771.1 |
| R:GCATAAGTAGGCATCGTATTTGTAT | |||
| TLR6 | F:CATCACCAGCCTCAAGCATTT | 90 | NM_213760.2 |
| R:TTCAGTTGTGTCAAGTTGCCAA | |||
| TLR7 | F:ATAGCGAGCATCACTCCAGCC | 127 | NM_001097434.1 |
| R:TAATCTGCTGCCTTCTGGTGC | |||
| TLR8 | F:CTGGGATGCTTGGTTCATCT | 150 | NM_214187.1 |
| R:CATGAGGTTGTCGATGATGG | |||
| TLR9 | F:ACAATGACATCCATAGCCGAGT | 80 | NM_213958.1 |
| R:CAGATCGTTGCCGCTAAAGT | |||
| PBD-1 | F:TGCCACAGGTGCCGATCT | 105 | NM_213838.1 |
| R:CTGTTAGCTGCTTAAGGAATAAAGGC | |||
| PR39 | F:CCACTCCATCACCGTTTTCC | 129 | NM_214450.1 |
| R:CAAGGCCACCTCCGTTTT | |||
| GAPDH | F:AGGTCGGAGTGAACGGATTTG | 118 | NM_001206359.1 |
| R:ACCATGTAGTGGAGGTCAATGAAG |
F, forward; R, reverse; PBD-1, porcine beta-defensin 1; PR39, proline-arginine rich 39-amino acid peptide; TLR, Toll-like receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.