TABLE 1.
Quantified proteins with an altered abundance after the consecutive incubation with capacitation medium and in vitro induction of the acrosome reaction.
| Gene name |
# Peptides quantified under strict criteria |
#Peptide differentially abundant |
DGC-CAP/CAP-AR/ DGC-AR |
Abundance alteration |
Related function |
| ACR | 10 | 4 | DGC-CAP; CAP-AR | ↓ | Fertilization |
| ACRBP | 17 | 8 | CAP-AR | ↓ | Fertilization |
| ACRV1 | 3 | 3 | DGC-CAP; CAP-AR | ↓ | Fertilization |
| AKAP3 | 16 | 12 | DGC-CAP | ↓ | Sperm motility |
| AKAP4 | 22 | 16 | DGC-CAP | ↓ | Sperm motility |
| ATP5F1A | 15 | 1 | DGC-CAP | ↑ | Energy production |
| ATP5F1D | 2 | 2 | CAP-AR | ↑ | Energy production |
| ATP5IF1 | 3 | 1 | DGC-AR | ↑ | Energy production |
| ATP5O | 1 | 1 | DGC-AR | ↑ | Energy production |
| CCT2 | 2 | 1 | DGC-AR | ↓ | Protein folding |
| ENO1 | 11 | 1 | DGC-AR | ↓ | Energy production |
| ERLIN2 | 1 | 1 | DGC-AR | ↑ | Signaling |
| FAM209A | 2 | 1 | DGC-CAP | ↑ | Unknown |
| FAM71B | 2 | 1 | DGC-AR | ↓ | RNA biogenesis |
| GGH | 5 | 2 | CAP-AR | ↓ | Metabolism of folic acid |
| GLUL | 3 | 1 | DGC-CAP | ↓ | Signaling |
| GSTM3 | 9 | 3 | DGC-AR | ↓ | Detoxification |
| HK1 | 19 | 2 | CAP-AR | ↓ | Energy production |
| HSPA2 | 18 | 3 | DGC-AR | ↓ | Protein folding |
| LYZL4 | 3 | 1 | DGC-AR | ↓ | Fertilization |
| LYZL6 | 1 | 1 | DGC-CAP | ↓ | Fertilization |
| ODF2 | 12 | 5 | DGC-CAP | ↓ | Sperm motility |
| PGAM2 | 6 | 1 | CAP-AR | ↓ | Sperm motility |
| PGK2 | 14 | 5 | DGC-CAP | ↓ | Sperm motility |
| PHB2 | 1 | 1 | DGC-CAP; CAP-AR | ↑ | Signaling |
| PSMA4 | 1 | 1 | CAP-AR | ↑ | Protein degradation |
| REEP5 | 1 | 1 | CAP-AR | ↑ | Signaling |
| ROPN1B | 1 | 1 | DGC-AR | ↓ | Sperm motility |
| RSPH1 | 1 | 1 | DGC-AR | ↑ | Sperm motility |
| SLC2A14 | 1 | 1 | DGC-AR | ↓ | Detoxification |
| SOD2 | 1 | 1 | DGC-AR | ↑ | Detoxification |
| SPACA3 | 5 | 2 | DGC-CAP | ↓ | Fertilization |
| SPACA7 | 1 | 1 | CAP-AR | ↓ | Fertilization |
| TMED10 | 3 | 1 | CAP-AR | ↑ | Vesicular trafficking |
| TUBA1A | 1 | 1 | DGC-CAP | ↑ | Sperm motility |
| ZPBP | 10 | 2 | CAP-AR | ↓ | Fertilization |
DGC-CAP: proteins differentially detected after incubation with capacitation medium; CAP-AR: proteins differentially detected after induction of the acrosome reaction; DGC-AR: proteins differentially detected after incubation of capacitation medium followed by the induction of the acrosome reaction; ↑: increased abundance; ↓: decreased abundance; Related function: according to public databases.