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. 2019 Nov 26;9:17573. doi: 10.1038/s41598-019-53973-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antigens identified from the NH group. (A) The mean IgG reactivity to antigens was compared between the NH and NL mean intensity among 28 antigens. Corresponding p value (Wilcoxon test, −log10 of p value) to each antigen is shown in grey line on the secondary y axis. (B) Sensitivity of top 5 proteins at specificity 0.967 and sensitivity/specificity for combined antigens. (C) Different profiles of comparison of infected groups with NL and NH as a reference. Upper: number of significantly reactive proteins identified in F + E− group in comparison with NL (left circle) and NH (right). Bottom: number of significantly reactive proteins identified in F + E+ group in comparison with NL (left circle) and NH (right).