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. 2019 Nov 26;9:17602. doi: 10.1038/s41598-019-54070-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fold change in mRNA of FOLR1 gene in maternal tissues. (a) Brain (F = 364.0, df = 8). (b) Liver (F = 25.91, df = 8). (c) Kidney (F = 113.6, df = 8). (d) Placenta (F = 112.6, df = 7), fetal tissues. (e) Liver (Male) (F = 48.5, df = 7), (Female) (F = 60.8, df = 7). (f) Brain (Male) (F = 11.2, df = 7), (Female) (F = 19.9, df = 7). (g) Kidney (Male) (F = 50.1, df = 6), (Female) (F = 46.5, df = 6) normalized with GAPDH. *p < 0.05, **p < 0.01, ***p < 0.001 vs BNFN, ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs BDFN, ^p < 0.05, ^^p < 0.01, ^^^p < 0.001 vs BOFN, ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 vs BNFD and ^&p < 0.05, ^&&p < 0.01, ^&&&p < 0.001 vs BNFO. The data is presented as mean ± SD^. (N = 4). B12 normal folate normal (BNFN), B12 normal folate over-supplemented (BNFO), B12 normal folate deficient (BNFD), B12 deficient folate normal (BDFN), B12 deficient folate over-supplemented (BDFO), B12 deficient folate deficient (BDFD), B12 over-supplemented folate normal (BOFN), B12 over-supplemented folate over-supplemented (BOFO), B12 over-supplemented folate deficient (BOFD).