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. 2019 Nov 26;9:17586. doi: 10.1038/s41598-019-53854-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Protein profile synthesized during kinetic growth of the GP543 strain grown in GYS medium. Samples of culture from a batch fermentation were taken every two hours until 28 hours of culture was reached. The samples were resolved by SDS-PAGE in duplicate: (A) protein profile in SDS-PAGE gel stained with Coomassie blue (B) Western blot analysis using a specific anti-S-layer antibody detected the production of the S-layer protein (around 100 kDa).