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. 2019 Nov 20;10:2449. doi: 10.3389/fimmu.2019.02449

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Copyright © 2019 Dubois-Camacho, Diaz-Jimenez, De la Fuente, Quera, Simian, Martínez, Landskron, Olivares-Morales, Cidlowski, Xu, Gao, Xie, Chnaiderman, Soto-Rifo, González, Calixto and Hermoso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Over-expression and inhibition of miR-378a-3p levels alter viability of colonocytes cell line. (A) IEC cells over-expressing the primiR-378, and (B) inhibiting the miR-378a-3p and their respective control cell lines (CTR PrimiR-378, CTR Inhibitor) were cultured by 6, 12, 24, 48, and 72 h. The number of viable cells were counted using trypan blue stain. The % of viability was achieved with PI stain by flow cytometry in (C) IEC cells over-expressing the primiR-378 and (D) inhibiting the miR-378a-3p cultured by 24, 48, and 72 h. Four experiments by duplicated were analyzed using Two-way ANOVA with Bonferroni multiple comparisons test were performed. *P = < 0.05, **P = < 0.01. ***P = < 0.001, ****P = < 0.0001.