FIGURE 1.

Tau mRNA interacts with mRNA-binding proteins and is expressed in SH-SY5Y cells. (A) SH-SY5Y cells were treated with RA for 4 days and immunostained with anti-tau antibody (Alexa Fluor 555) (left-hand image); the right-hand image shows a phase contrast (PC). (B) The cytosolic fraction of differentiated SH-SY5Y cells was immunoprecipitated with specific antibodies directed against dendritic mRNA-binding proteins, or control IgG. RNA was extracted from the immune complex, and double-stranded tau cDNA was detected by RT-PCR. (C) RNA was extracted from the cytosolic fraction and RT-PCR was performed using specific primers to examine the presence of the 3- or 4-repeat regions. The primer positions are shown as opposed arrows. φX174: molecular marker.