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. 2019 Nov 26;9:17604. doi: 10.1038/s41598-019-53898-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of EF on expression of lineage specific transcription factors. T cells were left unstimulated (control) or activated with anti CD3/CD28 antibodies (CD3/CD28). Following exposure to EFs (CD3/CD28 + EF), T cells were harvested and 3 days post EF stained for the lineage specific transcription factors (a) T-bet (Th1), (b) RORγt (Th17), (c) FoxP3 (Treg) and (d) GATA-3 (Th2). Data plotted represent individual donor values and right panels show the mean values ± SEM; n = 6 separate T cell cultures; *p < 0.05.