Figure 6.

EF exposure results in a decrease in STAT3 phosphorylation in T cells. T cell derived protein was isolated 18 hours following 4 h EF-exposure (150 mV/mm) and analysed for STAT3, pSTAT3 and β-actin expression levels by Western Blotting. p-STAT3:STAT3 band intensity, was determined by densitometry and expressed as the ratio of p-STAT3 to STAT3. Shown as mean ± SEM of independent experiments from 4 individual human T cell preparations; *p < 0.05. Blots shown are from the same gel. The blots were stripped after probing for p-STAT3 and reprobed with β-actin. The full-length blots are presented in Supplementary Figure 3.