Fig. 3. Sna acts down-stream of Hep in JNK-mediated cell death.

a–h Light micrographs showing Drosophila adult eyes. The small and rough eye phenotype resulted from ectopic expression of dTAK1 a or Hep^CA e is evidently suppressed by mutating one copy of endogenous bsk c and g, or knocking-down sna d and h, but not GFP b and f, which served as a negative control. i–p Light micrographs of Drosophila adult wings. Compared with the controls i and m, the wing phenotypes of Sd > Hep^WT j and ptc > Hep^WT n flies are suppressed by expressing a sna-IR l and p, but not GFP k and o. In m–p, the lower panels are high magnification of the boxed areas in upper panels. Statistical analysis of the adult wing size/wild type (WT) q (n = 10) and the ACV phenotype r (n = 20 for each genotype) as shown in figures i–l and m–p, respectively. One-way ANOVA with Bonferroni multiple comparison test was used to compute P-values, ***P < 0.001; ns, no significant difference. In all wings, anterior is to the left and distal up. See the electronic supplementary material for detailed genotypes. Scale bars: 100 μm in a–l and m–p (upper panels), 50 μm in m–p (lower panels).