Fig. 5. Sna regulates FoxO-mediated cell death.

Light micrographs of Drosophila adult eyes a–f, adult wings n–q, and s–v and fluorescence micrographs of third instar larval eye discs g–l are shown. The small eye phenotype and massive cell death, produced by GMR > Egr a and g, are obviously suppressed in heterozygous dFoxO^Δ94 background b and h or by knockdown of dFoxO c and i. Expression of dFoxO driven by GMR-GAL4 results in reduced eye size and increased cell death in eye discs d and j, which are suppressed by mutating one copy of endogenous sna e and k or RNAi-mediated depletion of sna f and l. Compared with the controls n and s, the wing phenotypes of Ser > dFoxO o and Sd > dFoxO t flies are partially suppressed by depletion of sna q and v, but not that of GFP p and u. In all wings, anterior is to the left and distal up. Statistical analysis of AO staining m, the adult wing size/wild type (WT) r and w as shown in figures g–l, n–q, and s–v, respectively (n = 10). One-way ANOVA with Bonferroni multiple comparison test was used to compute P-values, ***P < 0.001; ns, no significant difference. See the electronic supplementary material for detailed genotypes. Scale bar: 100 μm in a–f, n–q and s–v, 50 μm in g–l.