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. 2019 Nov 26;9:17625. doi: 10.1038/s41598-019-53875-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of deconvolution results either obtained using a measured PSF or a modelled PSF. (a1) 3D-reconstructions of a cleared fruit fly from 353 raw slices (MIP projection). Images were recorded using a 10x objective with NA = 0.3 (UPLFLN 10x, Olympus, Germany). (a2) Data from a1 after deconvolution with the measured PSF depicted in a2. (a3). Data from a1 after deconvolution with the modelled PSF depicted in a2. A comparison of a2 and a3 shows that the deconvolution results are almost identical. (a4) Mean standard deviations (MSD) measured between a1 vs. a2 (4.30 × 10⁵ = 99.8%), a1 vs. a3 (4.31 × 10⁵ = 100%) and a2 vs. a3 (9.33 × 10⁴ = 21.6%). (b1) Detail of a cleared mouse embryo (dorsal root ganglia) obtained from 419 raw slices (MIP projection). Images were recorded using a 20x objective with NA = 0.45 (LUCPLFLN, Olympus, Germany). (b2) Data from b1 after deconvolution using the measured PSF depicted in b2,3). Data from b1 after deconvolution using the modelled PSF depicted in b2 (right). Visual comparison of b2 and b3 confirms that the deconvolution results are virtually identical. (b4) Mean standard deviations (MSD) measured between b1 vs. b2 (1.37 × 10⁸ = 85.5%), b1 vs. b3 (1.61 × 10⁸ = 100%) and b2 vs. b3 (1.52 × 10⁶ = 0.95%).