Fig. 3.

Aβ42-vaccination normalized sleep−wake patterns, diurnal fluctuation of ISF Aβ, and Aβ plaque deposition in the hippocampus and striatum in APPswe/PS1δE9 mice. a, g Sleep−wake cycles in 9-month-old PBS-treated (a) and Aβ42-immunized (g) APPswe/PS1δE9 mice for 2 days shown as minutes awake per hour. d, j Comparison of minutes awake per hour between the dark and the light periods in each group. b, h Diurnal rhythms of ISF Aβ in the hippocampus of 9-month-old PBS-treated (b) and Aβ42-immunized (h) APPswe/PS1δE9 mice for 2 days. e, k Comparison of percent average of absolute values of ISF Aβ in the hippocampus between the dark and the light periods. c, i Diurnal rhythms of ISF Aβ in the striatum of 9-month-old PBS-vaccinated (c) and Aβ42-vaccinated (i) APPswe/PS1δE9 mice for 2 days. f, l Comparison of percent average of absolute values of ISF Aβ in the striatum between the dark and the light periods. m−p Representative brain sections of the hippocampus (m and o) and striatum (n and p) of mice from each group stained with Aβ antibody. q and r Amount of Aβ deposition in the PBS-treated mice and Aβ42-vaccinated mice are shown with amount of Aβ deposition in 6- and 9-month-old APPswe/PS1δE9 mice in the hippocampus (q) and striatum (r), N = 5−6 in each group; two-tailed t test.; *P < 0.05; ***P < 0.001; data shown are the means ± SEM. Adapted by permission from AAAS: Sci Transl Med [40]