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. 2019 Nov 20;10:1503. doi: 10.3389/fpls.2019.01503

Figure 3.

Figure 3

Integration of oligo painting FISH and existing cytogenetic markers on mitotic metaphase plates of Musa. (A) M. acuminata ssp. malaccensis “Pahang” (2n = 22, AA; chromosome 1 in red, BAC clone 2G17 in green). (B) M. schizocarpa “Schizocarpa” (2n = 22, SS; chromosome 2 in red, tandem repeat CL33 in green). (C) M. schizocarpa “Schizocarpa” (2n = 22, SS; short arm of chromosome 4 in green, 5S rRNA in red—two loci are localized on long arm of chromosome 4). (D) M. acuminata ssp. malaccensis “Pahang” (2n = 22, AA; 5S rRNA in red, short arm of chromosome 3 in green bears 5S rRNA). Chromosomes were counterstained with DAPI (blue). Bars = 5 µm. Arrows indicate colocalization of oligo painting FISH probes with existing cytogenetic markers.