Table 2. PCR reaction condition for MS4A6A, TREM2, and CD33 gene polymorphisms .

Reaction component	MS4A6A gene polymorphism	TREM2 gene polymorphism	CD33 gene polymorphism
WATER, Nuclease-free	17 µL	18 µL	17 µL
10x FastDigest or 10x FastDigest Green Buffer	2 µL	2 µL	2 µL
PCR reaction mixture	10 µL (0.2 µg DNA)	10 µL(0.1-0.5 µg DNA)	10 µL(0.2µg DNA)
FastDigest enzyme	1 µL (NlaIII)	1-2 µL (HinP1I)	1 µL (NlaIII)
Expexted size of digested products	67+174 bp	164+369 bp	282+366 bp