FIG 5.

Intracellular distribution of RNPs in RNP^tracker infected HAE. (A) Cells were infected with MeV-RNP^tracker and imaged at 72 hpi by confocal microscopy. The cells were fixed, permeabilized, and then immunostained for N protein (red). Nuclei were visualized with DAPI (blue). The left panel is a vertical section view, and the vertical bars indicate the plane of view for the series of en face images on the right. The right panels show maximum intensity projection images of three to five z-stacks at the indicated apical, circumapical, and basolateral regions. Scale bars, 20 μm. Images are representative from n = 6 samples (two technical replicates from three human donors [biological replicates]). (B) Colocalization between RNP^tracker and N protein within infectious centers was measured by using Mander’s colocalization coefficient.