FIG 2.
Pattern of Pdes-lacZ expression in the wild type and DesKPA mutants. Plasmids expressing different DesKPA mutants (DesKP16A, DesKP26A, DesKP135A, and DesKP148A) or wild-type DesK (WT) from the xylose-inducible promoter Pxyl, as well as with empty vector (EV), were transformed in the reporter strains, as indicated. (A) DAK3 transformants were streaked in LB plates in the presence of 60 μg/ml X-Gal and 0.05% xylose and incubated at 37°C (left) or for 5 h at 37°C and then transferred to 25°C for 24 h (right). (B) AKP20 transformants were grown in LB at 37°C in the absence (black bars) or presence (gray bars) of 0.05% xylose. Shown values correspond to the β-galactosidase activity of aliquots taken after 6 h of growth, expressed as a percentage of wild-type activity in the absence of xylose.