TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strain | ||
| Escherichia coli | ||
| DH5α | supE44 thi-1 ΔlacU169 (Φ80lacZΔM15) endA1 recA1 hsdR17 gyrA96 relA1 trp-6 cysT329::lac inmpI(209) | Laboratory stock |
| Bacillus subtilis | ||
| JH642 | trpC2 pheA1 | Laboratory stock |
| DAK3 | JH642 amyE::Pdes-lacZ desK::km Pxyl-desR; Cmr Kmr | 17 |
| AKP20 | JH642 amyE::Pdes-lacZ desK::km Pkm-desR; Cmr Kmr | 13 |
| Plasmid | ||
| pHPKS | B. subtilis replicative vector of low copy number, Ermr Lmr | 3 |
| pARD7 | pHPKS derivative with Pxyl promoter cloned in SacI | 35 |
| pARD8 | pHPKS derivative with Pxyl promoter cloned in SacI and desKC end in SmaI/PstI | 35 |