TABLE 2.
Oligonucleotides used in this study
| Name | Sequencea |
|---|---|
| DesKBamHI33Up | AGTAACATGGATCCCAGAAAATGAGGTAAGATC |
| DesKPstILw | GCTGATCTTCTGCAGTAAATATACTAATC |
| DesKSmaLw | GGAGATCCCGGGCAATTCGCTGACG |
| Mutagenic oligonucleotides | |
| P16A Up | TAAACGGGATTACGGCTTATATATGGAC |
| P16A Lw | CATATATAAGCCGTAATCCCGTTTAG |
| P26A Up | ATTTTTCATCCTCGCTTTCTACTTTATA |
| P26A Lw | AAGTAGAAAGCGAGGATGAAAAATATC |
| P135A Up | AAATTGCTTTTGTCGTTATTACCCTCAT |
| P135A Lw | AAAAGCAATTTGTGTCAGAAAGAATTC |
| P148A Up | CGCAATTTTATTGGCTTTCAGTATAA |
| P148A Lw | TTATACTGAAAGCCAATAAAATTGC |
| P103A Up | ATTAAGGAACGCGTCGCTTTTCATATT |
| P103A Lw | ATAAAATATGAAAAGCGACGCGTTCCTTAAT |
| L174P Up | GGATTGCAGAACCGGTAAAATTAGAAG |
| L174P Lw | TTCTTCTAATTTTACCGGTTCTGCAATCC |
| A172P Up | ATGAACGGATTCCAGAACTGGT |
| A172P Lw | TTACCAGTTCTGGAATCCGTTC |
| A167P Up | AGCTCGAGGATCCAAATGAACGG |
| A167P Lw | ATCCGTTCATTTGGATCCTCGAGC |
| L160P Up | GCGCGAACGACCTGAAGAAAAGC |
| L160P Lw | TCGAGCTTTTCTTCAGGTCGTTCGC |
| S149 F150 Up | TTTATTGCCCAGTTTCATAAAAAGCCG |
| S149 F150 Lw | GGCTTTTTATGAAACTGGGCAATAAAATTG |
a
Underlining indicates restriction sites.