FIG 2.

Binding of PxHsp90 to Cry1Ab and Cry1Ac toxins. (A to C) ELISAs of binding of Cry1Ab protoxin (A) or Cry1Ac protoxin (B and C) to PxHsp90. ELISA plates were coated with 0.5 μg of each protoxin, and after washing of unbound proteins, the plates were incubated with different concentrations of PxHsp90, which was revealed with anti-His antibody as described in Materials and Methods. Panel C shows an ELISA of binding of a nonsaturating concentration of PxHsp90 (100 nM) to Cry1Ac with 1 mM ATP (lane 1), without ATP (lane 2), or with 20 μM geldanamycin (Hsp90 inhibitor) containing 1 mM ATP (lane 3). (D) Binding of PxHsp90 (300 nM) to 0.5 μg Cry1Ac protoxin or 0.5 μg activated toxin.