FIG 3.

PxHsp90 assists Cry1Ab protoxin in binding to the CAD receptor. (A) P. xylostella mortality assays using 35 ng/cm² of Cry1AbR99E, Cry1AbE129K, or Cry1AbG439D protoxin in the absence and presence of 1 μg PxHsp90. Data represent means of results from three replicates of 24 larvae per treatment with standard deviations. (B) ELISAs of binding of Cry1Ab or Cry1AbG439D protoxin and a mixture of Cry1AbG439D protoxin-PxHsp90 at a 1:30 ratio (protoxin to chaperone) to CAD CR12. (C) ELISAs of binding of Cry1Ab or Cry1AbG439D activated toxin and a mixture of Cry1AbG439D activated toxin-PxHsp90 at a 1:30 ratio (toxin to chaperone) to CAD CR12. ELISA plates were coated with 0.5 μg of CR12. After washing, the toxin bound to CR12 was revealed with an anti-Cry1Ab antibody as described in Materials and Methods.