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. 2019 Nov 26;10(6):e02775-19. doi: 10.1128/mBio.02775-19

FIG 4.

FIG 4

PxHsp90 protects Cry1Ab protoxin from protease degradation. (A) Treatment of Cry1Ab protoxin with midgut juice from P. xylostella as described in Materials and Methods. One microgram of protoxin was treated with the gut proteases for different times in the presence of either BSA or PxHsp90 and revealed by Western blotting using the anti-Cry1Ab antibody. (B) Treatment of Cry1Ab protoxin for 1 h with different concentrations of trypsin in the presence of either BSA or PxHsp90. The protoxin was revealed by Western blotting using an anti-Cry1Ab antibody. Control Cry1Ab protoxin without protease treatment is shown in the first lane. Representative results from three replicates are shown. The arrows indicate the positions of the 130-kDa Cry1Ab protoxin and the 60-kDa activated toxin.