Figure 1.

TLR2/4 are involved in COX-dependent PGE₂ synthesis in subcortical tissues. (a) Schedule of experiments in this study. After social isolation for 1 week, a male wild-type or TLR-DKO mouse was subjected to SDS through an encounter with an ICR aggressor mouse for 10 min daily for 1 day (Day 1) or 10 consecutive days (Day 1 through Day 10). The PGE₂ measurement was performed immediately after the SDS exposure on Day 1 or Day 10, or at 24 h after the last SDS exposure (i.e. on Day 11). (b,c) The PGE₂ contents in subcortical (b) and cortical (c) tissues of wild-type (WT), TLR-DKO and COX1-KO mice without SDS (naïve), immediately after single SDS (S-SDS), and immediately and at 24 h after the last SDS session of repeated SDS (R-SDS). (d,e) The PGE₂ contents in subcortical (d) and cortical (e) tissues of wild-type mice administrated with SC-560 and SC-236 that are COX1 and COX2 inhibitors, respectively, as well as vehicle without SDS and immediately after the last session of repeated SDS (R-SDS). *P < 0.05, **P < 0.01 for Bonferroni’s multiple comparisons test. Data are shown as means ± SEM.